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far-red cy5 dye  (KARL STORZ)

 
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    KARL STORZ far-red cy5 dye
    Far Red Cy5 Dye, supplied by KARL STORZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/far-red+cy5+dye/far+red+cy5+dye/pm37371522-253-5-20
    Average 90 stars, based on 1 article reviews
    far-red cy5 dye - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery.
    Article Snippet: To this end, the far-red Cy5 dye (lex = 650 nm, lem = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [40,45], while the nearinfrared Cy7 dye (lex = 750 nm, lem = 777 nm; [16]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery
    Article Snippet: To this end, the far-red Cy5 dye (l ex = 650 nm, l em = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [ , ], while the near-infrared Cy7 dye (l ex = 750 nm, l em = 777 nm; [ ]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Flow Cytometry:

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery.
    Article Snippet: To this end, the far-red Cy5 dye (lex = 650 nm, lem = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [40,45], while the nearinfrared Cy7 dye (lex = 750 nm, lem = 777 nm; [16]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery
    Article Snippet: To this end, the far-red Cy5 dye (l ex = 650 nm, l em = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [ , ], while the near-infrared Cy7 dye (l ex = 750 nm, l em = 777 nm; [ ]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Binding Assay:

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery.
    Article Snippet: To this end, the far-red Cy5 dye (lex = 650 nm, lem = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [40,45], while the nearinfrared Cy7 dye (lex = 750 nm, lem = 777 nm; [16]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery
    Article Snippet: To this end, the far-red Cy5 dye (l ex = 650 nm, l em = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [ , ], while the near-infrared Cy7 dye (l ex = 750 nm, l em = 777 nm; [ ]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Staining:

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery.
    Article Snippet: To this end, the far-red Cy5 dye (lex = 650 nm, lem = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [40,45], while the nearinfrared Cy7 dye (lex = 750 nm, lem = 777 nm; [16]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery
    Article Snippet: To this end, the far-red Cy5 dye (l ex = 650 nm, l em = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [ , ], while the near-infrared Cy7 dye (l ex = 750 nm, l em = 777 nm; [ ]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Imaging:

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery.
    Article Snippet: To this end, the far-red Cy5 dye (lex = 650 nm, lem = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [40,45], while the nearinfrared Cy7 dye (lex = 750 nm, lem = 777 nm; [16]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery
    Article Snippet: To this end, the far-red Cy5 dye (l ex = 650 nm, l em = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [ , ], while the near-infrared Cy7 dye (l ex = 750 nm, l em = 777 nm; [ ]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Expressing:

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery.
    Article Snippet: To this end, the far-red Cy5 dye (lex = 650 nm, lem = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [40,45], while the nearinfrared Cy7 dye (lex = 750 nm, lem = 777 nm; [16]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery
    Article Snippet: To this end, the far-red Cy5 dye (l ex = 650 nm, l em = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [ , ], while the near-infrared Cy7 dye (l ex = 750 nm, l em = 777 nm; [ ]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Concentration Assay:

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery.
    Article Snippet: To this end, the far-red Cy5 dye (lex = 650 nm, lem = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [40,45], while the nearinfrared Cy7 dye (lex = 750 nm, lem = 777 nm; [16]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery
    Article Snippet: To this end, the far-red Cy5 dye (l ex = 650 nm, l em = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [ , ], while the near-infrared Cy7 dye (l ex = 750 nm, l em = 777 nm; [ ]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Sequencing:

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery.
    Article Snippet: To this end, the far-red Cy5 dye (lex = 650 nm, lem = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [40,45], while the nearinfrared Cy7 dye (lex = 750 nm, lem = 777 nm; [16]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.

    Article Title: A Truncated 14-Amino-Acid Myelin Protein-Zero-Targeting Peptide for Fluorescence-Guided Nerve-Preserving Surgery
    Article Snippet: To this end, the far-red Cy5 dye (l ex = 650 nm, l em = 667 nm) has demonstrated compatibility with a KARL STORZ prototype [ , ], while the near-infrared Cy7 dye (l ex = 750 nm, l em = 777 nm; [ ]) is compatible with the Image 1 S Rubina (KARL STORZ) and the Firefly endoscope+ (Intuitive) set-up.



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    ( a ) Protection of siRNA against serum nucleases. Free siRNA and GNC–siRNA (100 nM siRNA) were incubated within 10% serum for multiple time points, and analysed by polyacrylamide gel electrophoresis. ( b ) Cellular uptake of free siRNA and GNC–siRNA into Panc-1 cells. siRNA was labelled with <t>Cy5</t> dye (Cy5-siRNA), the Panc-1 cells were incubated with various Cy5-siRNA formulations for 1 h and observed by confocal microscope with a 633 nm laser excitation. Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bars, 20 μm. ( c ) Lysosomal escape of GNC–siRNA in Panc-1 cells. The lysosomes of cells were stained with LysoTracker Green for 1 h, and the Panc-1 cells were treated with GNC–Cy5-siRNA for 1 h. The cells were observed by confocal microscope over different time points. Scale bars, 20 μm. ( d ) Expression level of NGF mRNA in Panc-1 cells analysed by RT–PCR, the dotted line referred to the expression level of NGF mRNA in Panc-1 cells transfected with commercially available Lipofectamine 2000 transfection agent (Lipo2000-siRNA), which served as a positive control. Mean±s.d. ( n =3). * P <0.01 compared with the nontreated control; Student's t -test. ( e ) Expression level of NGF protein in Panc-1 cells evaluated by western blotting. GNC binding with nsRNA was labelled as GNC–nsRNA and served as control siRNA.
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    Image Search Results


    ( a ) Protection of siRNA against serum nucleases. Free siRNA and GNC–siRNA (100 nM siRNA) were incubated within 10% serum for multiple time points, and analysed by polyacrylamide gel electrophoresis. ( b ) Cellular uptake of free siRNA and GNC–siRNA into Panc-1 cells. siRNA was labelled with Cy5 dye (Cy5-siRNA), the Panc-1 cells were incubated with various Cy5-siRNA formulations for 1 h and observed by confocal microscope with a 633 nm laser excitation. Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bars, 20 μm. ( c ) Lysosomal escape of GNC–siRNA in Panc-1 cells. The lysosomes of cells were stained with LysoTracker Green for 1 h, and the Panc-1 cells were treated with GNC–Cy5-siRNA for 1 h. The cells were observed by confocal microscope over different time points. Scale bars, 20 μm. ( d ) Expression level of NGF mRNA in Panc-1 cells analysed by RT–PCR, the dotted line referred to the expression level of NGF mRNA in Panc-1 cells transfected with commercially available Lipofectamine 2000 transfection agent (Lipo2000-siRNA), which served as a positive control. Mean±s.d. ( n =3). * P <0.01 compared with the nontreated control; Student's t -test. ( e ) Expression level of NGF protein in Panc-1 cells evaluated by western blotting. GNC binding with nsRNA was labelled as GNC–nsRNA and served as control siRNA.

    Journal: Nature Communications

    Article Title: Gold nanoclusters-assisted delivery of NGF siRNA for effective treatment of pancreatic cancer

    doi: 10.1038/ncomms15130

    Figure Lengend Snippet: ( a ) Protection of siRNA against serum nucleases. Free siRNA and GNC–siRNA (100 nM siRNA) were incubated within 10% serum for multiple time points, and analysed by polyacrylamide gel electrophoresis. ( b ) Cellular uptake of free siRNA and GNC–siRNA into Panc-1 cells. siRNA was labelled with Cy5 dye (Cy5-siRNA), the Panc-1 cells were incubated with various Cy5-siRNA formulations for 1 h and observed by confocal microscope with a 633 nm laser excitation. Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bars, 20 μm. ( c ) Lysosomal escape of GNC–siRNA in Panc-1 cells. The lysosomes of cells were stained with LysoTracker Green for 1 h, and the Panc-1 cells were treated with GNC–Cy5-siRNA for 1 h. The cells were observed by confocal microscope over different time points. Scale bars, 20 μm. ( d ) Expression level of NGF mRNA in Panc-1 cells analysed by RT–PCR, the dotted line referred to the expression level of NGF mRNA in Panc-1 cells transfected with commercially available Lipofectamine 2000 transfection agent (Lipo2000-siRNA), which served as a positive control. Mean±s.d. ( n =3). * P <0.01 compared with the nontreated control; Student's t -test. ( e ) Expression level of NGF protein in Panc-1 cells evaluated by western blotting. GNC binding with nsRNA was labelled as GNC–nsRNA and served as control siRNA.

    Article Snippet: For better observation, NGF siRNA was labelled with a far-red fluorescent dye Cy5 (excitation/emission at 649/670nm) at the 5′-end of the sense strand (Cy5-siRNA) (Ribobio, Guangzhou).

    Techniques: Incubation, Polyacrylamide Gel Electrophoresis, Microscopy, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Transfection, Positive Control, Control, Western Blot, Binding Assay

    ( a ) The circulation time of free siRNA and GNC–siRNA complex in blood. Cy5 dye-labelled siRNA (Cy5-siRNA) was used for visualization. Blood was drawn from mice after tail vein injection with various Cy5-siRNA formulations (30 μg Cy5-siRNA per mouse equivalent) at different time and imaged under a fluorescence imaging system. The fluorescence intensity of 0 h in each group was normalized as 100%. ( b ) In vivo tumour targeting. Balb/c nude mice with subcutaneous Panc-1 tumours were injected with different Cy5-siRNA formulations (30 μg Cy5-siRNA per mouse equivalent) via tail vein. After 6 h, fluorescence images of the mice were acquired with in vivo fluorescence imaging system, the white circles indicated the regions of subcutaneous tumours. ( c ) Tumour targeting by ex vivo imaging. After 24 h of Cy5-siRNA injection, major organs and tumours were isolated from mice for ex vivo fluorescence imaging. ( d ) The fluorescence intensity of Cy5-siRNA in major organs and tumours at 24 h after intravenous injection. * P <0.01 compared with free siRNA. ( e ) The concentration of gold in the major organs and tumours (expressed as % of given dose) at 6 and 24 h post injection of GNC–siRNA complex by ICP-MS. Fluorescence images and ICP-MS analysis confirmed the accumulation of GNC–siRNA complex into the tumour sites. Mean±s.d. ( n =4). * P <0.01; Student's t -test.

    Journal: Nature Communications

    Article Title: Gold nanoclusters-assisted delivery of NGF siRNA for effective treatment of pancreatic cancer

    doi: 10.1038/ncomms15130

    Figure Lengend Snippet: ( a ) The circulation time of free siRNA and GNC–siRNA complex in blood. Cy5 dye-labelled siRNA (Cy5-siRNA) was used for visualization. Blood was drawn from mice after tail vein injection with various Cy5-siRNA formulations (30 μg Cy5-siRNA per mouse equivalent) at different time and imaged under a fluorescence imaging system. The fluorescence intensity of 0 h in each group was normalized as 100%. ( b ) In vivo tumour targeting. Balb/c nude mice with subcutaneous Panc-1 tumours were injected with different Cy5-siRNA formulations (30 μg Cy5-siRNA per mouse equivalent) via tail vein. After 6 h, fluorescence images of the mice were acquired with in vivo fluorescence imaging system, the white circles indicated the regions of subcutaneous tumours. ( c ) Tumour targeting by ex vivo imaging. After 24 h of Cy5-siRNA injection, major organs and tumours were isolated from mice for ex vivo fluorescence imaging. ( d ) The fluorescence intensity of Cy5-siRNA in major organs and tumours at 24 h after intravenous injection. * P <0.01 compared with free siRNA. ( e ) The concentration of gold in the major organs and tumours (expressed as % of given dose) at 6 and 24 h post injection of GNC–siRNA complex by ICP-MS. Fluorescence images and ICP-MS analysis confirmed the accumulation of GNC–siRNA complex into the tumour sites. Mean±s.d. ( n =4). * P <0.01; Student's t -test.

    Article Snippet: For better observation, NGF siRNA was labelled with a far-red fluorescent dye Cy5 (excitation/emission at 649/670nm) at the 5′-end of the sense strand (Cy5-siRNA) (Ribobio, Guangzhou).

    Techniques: Injection, Fluorescence, Imaging, In Vivo, Ex Vivo, Isolation, Concentration Assay